ProSci

PVRL2 Antibody

Product Code:
 
PSI-19-627
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using PVRL2 antibody (19-627) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 2
Immunofluorescence analysis of HeLa cells using PVRL2 antibody (19-627). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PVRL2 antibody (19-627) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunofluorescence analysis of HeLa cells using PVRL2 antibody (19-627). Blue: DAPI for nuclear staining.

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PSI-19-627-100uL100 uL£565.00
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Further Information

Additional Names:
CD112, HVEB, PRR2, PVRL2, PVRR2, nectin-2, herpesvirus entry protein B, poliovirus receptor-like 2, poliovirus receptor-related 2 (herpesvirus entry mediator B)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 382-479 of human PVRL2 (NP_002847.1).
NCBI Gene ID #:
5819
NCBI Official Name:
nectin cell adhesion molecule 2
NCBI Official Symbol:
PVRL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
Q92692
User NOte:
Optimal dilutions for each application to be determined by the researcher.