ProSci

FBXL5 Antibody

Product Code:
 
PSI-19-751
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using FBXL5 antibody (19-751) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using FBXL5 antibody (19-751) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded human colon carcinoma using FBXL5 antibody (19-751) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using FBXL5 antibody (19-751) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using FBXL5 antibody (19-751) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FBXL5 antibody (19-751) at dilution of 1:100 (40x lens).

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PSI-19-751-50uL50uL£433.00
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Further Information

Additional Names:
FBXL5, FBL4, FBL5, FLR1
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1).
NCBI Gene ID #:
26234
NCBI Official Name:
F-box and leucine-rich repeat protein 5
NCBI Official Symbol:
FBXL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 79kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9UKA1
User NOte:
Optimal dilutions for each application to be determined by the researcher.