ProSci

LC3A/LC3B Antibody

Product Code:
 
PSI-19-759
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (19-759) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
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Immunofluorescence analysis of HeLa cells using LC3A/LC3B antibody (19-759).
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Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (19-759) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunofluorescence analysis of HeLa cells using LC3A/LC3B antibody (19-759).

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PSI-19-759-50uL50uL£433.00
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Further Information

Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human LC3A (NP_115903.1).
NCBI Gene ID #:
84557/81631
NCBI Official Symbol:
MAP1LC3A/MAP1LC3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q9GZQ8/Q9H492
User NOte:
Optimal dilutions for each application to be determined by the researcher.