ProSci

DOK1 Antibody

Product Code:
 
PSI-19-794
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using DOK1 antibody (19-794) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using DOK1 antibody (19-794). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using DOK1 antibody (19-794) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DOK1 antibody (19-794). Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-19-794-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
DOK1, MGC117395, MGC138860, P62DOK
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DOK1 (NP_001372.1).
NCBI Gene ID #:
1796
NCBI Official Name:
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
NCBI Official Symbol:
DOK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Obesity, Signal Transduction
Swissprot #:
Q99704
User NOte:
Optimal dilutions for each application to be determined by the researcher.