ProSci

SIRT3 Antibody, KO Validated

Product Code:
 
PSI-19-812
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of SW620 cells, using SIRT3 antibody (19-812) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
2 / 5
Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) HeLa cells, using SIRT3 antibody (19-812) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
3 / 5
Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (19-812) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human liver cancer using SIRT3 antibody (19-812) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded mouse brain using SIRT3 antibody (19-812) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of SW620 cells, using SIRT3 antibody (19-812) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) HeLa cells, using SIRT3 antibody (19-812) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (19-812) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SIRT3 antibody (19-812) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SIRT3 antibody (19-812) at dilution of 1:100 (40x lens).

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PSI-19-812-50uL50uL£505.00
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Further Information

Additional Names:
SIRT3 Antibody: SIR2L3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, hSIRT3
Application Note:
WB: 1:500 - 1:1000

IHC: 1:20 - 1:50
Background:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
NCBI Gene ID #:
23410
NCBI Official Name:
sirtuin 3
NCBI Official Symbol:
SIRT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9NTG7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.