ProSci

RUVBL1 Antibody

Product Code:
 
PSI-19-815
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using RUVBL1 antibody (19-815) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
2 / 5
Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody (19-815) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1 Antibody (19-815) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1 Antibody (19-815) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of A549 cells using RUVBL1 antibody (19-815). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using RUVBL1 antibody (19-815) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody (19-815) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1 Antibody (19-815) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1 Antibody (19-815) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using RUVBL1 antibody (19-815). Blue: DAPI for nuclear staining.

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PSI-19-815-50uL50uL£433.00
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Further Information

Additional Names:
RUVBL1, ECP54, NMP238, Pontin52, RVB1, TIH1, TIP49, TIP49A, INO80H, PONTIN
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:50
Background:
This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RUVBL1 (NP_003698.1).
NCBI Gene ID #:
8607
NCBI Official Name:
RuvB-like AAA ATPase 1
NCBI Official Symbol:
RUVBL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y265
User NOte:
Optimal dilutions for each application to be determined by the researcher.