ProSci

HELLS Antibody, KO Validated

Product Code:
 
PSI-19-887
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using HELLS antibody (19-887) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (19-887) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
3 / 4
Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (19-887) at dilution of 1:100 (40x lens).
4 / 4
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody (19-887). Western blot was performed from the immunoprecipitate using HELLS antibody (19-887) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using HELLS antibody (19-887) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (19-887) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (19-887) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody (19-887). Western blot was performed from the immunoprecipitate using HELLS antibody (19-887) at a dilition of 1:1000.

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PSI-19-887-50uL50uL£505.00
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Further Information

Additional Names:
ICF4, LSH, Nbla10143, PASG, SMARCA6, lymphoid-specific helicase, SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6, proliferation-associated SNF2-like protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).
NCBI Gene ID #:
3070
NCBI Official Name:
helicase, lymphoid specific
NCBI Official Symbol:
HELLS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9NRZ9
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.