ProSci

EIF2S3 Antibody

Product Code:
 
PSI-22-329
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using EIF2S3 antibody (22-329) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver using EIF2S3 antibody (22-329) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human stomach using EIF2S3 antibody (22-329) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded rat brain using EIF2S3 antibody (22-329) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using EIF2S3 antibody (22-329) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using EIF2S3 antibody (22-329) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EIF2S3 antibody (22-329) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EIF2S3 antibody (22-329) at dilution of 1:100 (40x lens).

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PSI-22-329-50uL50uL£433.00
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Further Information

Additional Names:
EIF2S3, EIF2, EIF2G, EIF2gamma, eIF-2gA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_001406.1).
NCBI Gene ID #:
1968
NCBI Official Name:
eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
NCBI Official Symbol:
EIF2S3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P41091
User NOte:
Optimal dilutions for each application to be determined by the researcher.