ProSci

SECISBP2 Antibody

Product Code:
 
PSI-22-448
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using SECISBP2 antibody (22-448) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
2 / 4
Immunohistochemistry of paraffin-embedded human liver damage using SECISBP2 antibody (22-448) at dilution of 1:200 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human colon carcinoma using SECISBP2 antibody (22-448) at dilution of 1:200 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using SECISBP2 antibody (22-448).
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Western blot analysis of extracts of various cell lines, using SECISBP2 antibody (22-448) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
Immunohistochemistry of paraffin-embedded human liver damage using SECISBP2 antibody (22-448) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SECISBP2 antibody (22-448) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using SECISBP2 antibody (22-448).

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PSI-22-448-50uL50uL£433.00
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Further Information

Additional Names:
SECISBP2, SECIS binding protein 2, RP11-89K14.1, DKFZp686C09169, SBP2, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human SECISBP2 (NP_076982.3).
NCBI Gene ID #:
79048
NCBI Official Name:
SECIS binding protein 2
NCBI Official Symbol:
SECISBP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
Q96T21
User NOte:
Optimal dilutions for each application to be determined by the researcher.