ProSci

SRP19 Antibody

Product Code:
 
PSI-22-461
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using SRP19 antibody (22-461) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat ovary using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human vermiform appendix using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using SRP19 antibody (22-461). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SRP19 antibody (22-461) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SRP19 antibody (22-461). Blue: DAPI for nuclear staining.

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PSI-22-461-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
SRP19,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SRP19 (NP_003126.1).
NCBI Gene ID #:
6728
NCBI Official Name:
signal recognition particle 19kDa
NCBI Official Symbol:
SRP19
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P09132
User NOte:
Optimal dilutions for each application to be determined by the researcher.