ProSci

NFU1 Antibody

Product Code:
 
PSI-22-695
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using NFU1 antibody (22-695) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse intestine using NFU1 Antibody (22-695) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse brain using NFU1 Antibody (22-695) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using NFU1 antibody (22-695) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse intestine using NFU1 Antibody (22-695) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NFU1 Antibody (22-695) at dilution of 1:100 (40x lens).

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PSI-22-695-50uL50uL£433.00
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Further Information

Additional Names:
CGI-33, HIRIP, HIRIP5, MMDS1, NIFUC, Nfu, NifU, NFU1 iron-sulfur cluster scaffold homolog, mitochondrial, HIRA-interacting protein 5, NifU-like C-terminal domain containing, iron-sulfur cluster scaffold protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NFU1 (NP_001002755.1).
NCBI Gene ID #:
27247
NCBI Official Name:
NFU1 iron-sulfur cluster scaffold
NCBI Official Symbol:
NFU1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25-28kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UMS0
User NOte:
Optimal dilutions for each application to be determined by the researcher.