ProSci

L3MBTL3 Antibody

Product Code:
 
PSI-22-838
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using L3MBTL3 antibody (22-838) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
2 / 3
Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (22-838). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody (22-838). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (22-838) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using L3MBTL3 antibody (22-838) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (22-838). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody (22-838). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (22-838) at a dilition of 1:1000.

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PSI-22-838-50uL50uL£440.00
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Further Information

Additional Names:
MBT-1, MBT1, lethal(3)malignant brain tumor-like protein 3, H-l(3)mbt-like protein 3, L3MBTL3, histone methyl-lysine binding protein, l(3)mbt-like 3
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).
NCBI Gene ID #:
84456
NCBI Official Name:
L3MBTL histone methyl-lysine binding protein 3
NCBI Official Symbol:
L3MBTL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Stem Cell
Swissprot #:
Q96JM7
User NOte:
Optimal dilutions for each application to be determined by the researcher.