ProSci

KDM4A Antibody

Product Code:
 
PSI-23-306
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using KDM4A antibody (23-306) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse liver using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using KDM4A antibody (23-306) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).

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PSI-23-306-50uL50uL£433.00
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Further Information

Additional Names:
JMJD2A Antibody: JMJD2, JHDM3A, JMJD2A, TDRD14A, JMJD2, KIAA0677, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2).
NCBI Gene ID #:
9682
NCBI Official Name:
lysine (K)-specific demethylase 4A
NCBI Official Symbol:
KDM4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75164
User NOte:
Optimal dilutions for each application to be determined by the researcher.