ProSci

AKT1 (phospho S473) Antibody [H4]

Product Code:
 
PSI-54-321
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Recombinant Monoclonal Rabbit IgG / light chain kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
H4
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at 4°C for up to 6 months. Store at -20°C long term
1 / 2
Antibody: 54-321 at 1/1000 dilution<br>Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30ug/lane<br>Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/60 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
2 / 2
Antibody: 54-321 at 1/1000 dilution<br>Sample: Lane 1: 1ug of PDGF treated NIH-3T3 cell lysate . Lane 2: 500ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads<br>Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/66 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
❮❯

Antibody: 54-321 at 1/1000 dilution<br>Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30ug/lane<br>Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/60 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
Antibody: 54-321 at 1/1000 dilution<br>Sample: Lane 1: 1ug of PDGF treated NIH-3T3 cell lysate . Lane 2: 500ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads<br>Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/66 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-54-321-100ug100ug£657.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Application Note:
WB, IP dilution (1:1000 ? 1: 5000).
Buffer:
49% PBS, 50% Glycerol, 0.05% BSA, pH 7.2, 0.02% NaN3
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Also recommended for detection of phosphor-AKT1 in sheep & zebrafish
Immunogen:
Synthetic peptides derived from human AKT1 (RPHFPQFsYSASGTA)
Modifications:
Phospho-Specific
NCBI Gene ID #:
207
NCBI Official Name:
AKT serine/threonine kinase 1
NCBI Official Symbol:
AKT1
NCBI Organism:
homo sapiens
Purification:
Protein A purified
Research Area:
Signal Transduction, Cancer, Obesity
Swissprot #:
P31749
User NOte:
Optimal dilutions for each application to be determined by the researcher.