ProSci

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3867]

Product Code:
 
PSI-35-721
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3867
Regulatory Status:
 
RUO
Target Species:
 
Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Shipping:
 
blue ice or RT
Storage:
 
Coronavirus Nucleocapsid Antibody can be stored at -20˚C, stable for one year.
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<strong>Figure 1 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-721 and 35-722. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-721, 2ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-722, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng
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<strong>Figure 1 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-721 and 35-722. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-721, 2ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-722, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng

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PSI-35-721-0.1mg0.1mg£638.00
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Further Information

Additional Names:
Coronavirus Nucleocapsid, SARS
Application Note:
Suggested starting ranges are 1:10-1:50 for IFA and 1:20-1:200 for ELISA. BEST ELISA pairs (capture-conjugate): clones 3865-3867
Buffer:
Coronavirus Nucleocapsid Antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Physical State:
Liquid
Purification:
Coronavirus Nucleocapsid Antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Research Area:
Infectious Disease, COVID-19
SPECIFICITY:
Specific for the nucleocapsid of Covid-19 virus. Cross reacts with SARS. These antibodies are non-reactive with: Influenza A, Influenza B, Adenovirus and MERS.
User NOte:
Optimal dilutions for each application to be determined by the researcher.