ProSci

SARS-CoV-2 (COVID-19) Spike S1 Antibody [SP185]

Product Code:
 
PSI-10-351
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SP185
Regulatory Status:
 
RUO
Target Species:
 
Virus
Shipping:
 
blue ice or RT
Storage:
 
SARS-CoV-2 (COVID-19) Spike S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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<strong> Figure 1 Detection of SARS-CoV-2 Variant Proteins with Spike S1 Antibodies by Direct ELISA</strong> Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4 ˚ C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 10-351, 0.1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. <strong>Spike S1 antibody (10-351) cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7). </strong>
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<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 10-706. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (10-706, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate (PM6727) at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng
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<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9083-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9083-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng
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<strong>Figure 5 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9091-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9091-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 3.14 ng
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<strong> Figure 1 Detection of SARS-CoV-2 Variant Proteins with Spike S1 Antibodies by Direct ELISA</strong> Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4 ˚ C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 10-351, 0.1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. <strong>Spike S1 antibody (10-351) cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7). </strong>
<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 10-706. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (10-706, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate (PM6727) at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng
<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9083-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9083-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng
<strong>Figure 5 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9091-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9091-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 3.14 ng

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PSI-10-351-0.1mg0.1mg£638.00
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Further Information

Additional Names:
SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Buffer:
PBS pH7.2
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant SARS-CoV-2 spike protein, S1 subunit.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Purification:
The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.