SARS-CoV-2 (COVID-19) Helicase Recombinant Protein

SARS-CoV 2 Helicase; SARS-CoV 2 nsp13

This recombinant protein can be used for biological assays. For research use only.

The non?structural protein 13 (nsp13) of SARS?CoV 2 is a helicase that separates double?stranded RNA or DNA with a 5'?3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5?-3? direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom?s syndrome, Werner?s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.

Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.

6His tag at the C-terminus

43740578

ORF1a polyprotein;ORF1ab polyprotein

ORF1ab

Severe acute respiratory syndrome coronavirus 2

Liquid

YP_009725297.1

Greater than 80% as determined by reducing SDS-PAGE.

Thr32-Gly402

E. coli

P0DTC1