Rabbit anti Mouse IgE (Fc specific) conjugated with TRITC
Product Code:
RAM_IgE(Fc)_TRITC
Antibody Clonality:
Polyclonal
Storage:
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use an aliquot is thawed slowly in the dark at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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This product comes from:
Netherlands.
Typical lead time:
7-10 working days.
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Further Information
Applications Description:
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
The reactivity of the antiserum is directed to IgE as tested against mouse sera and a panel of purified homogenous immunoglobulins. The precipitation reaction obtained against serum containing monoclonal IgE shows a reaction of full identity with the precipitation reaction with the immunogen. No reaction is obtained with normal mouse serum. To identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Fluorochrome-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Fluorochrome-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
TRITC
Pools of highly purified homogenous IgE isolated from mouse serum. Freund?s complete adjuvant is used in the first step of the immunization procedure.
Tetramethylrhodamine isothiocyanate?conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse IgE, Fc specific