Nordic MuBio

Swine anti Rabbit IgG (heavy and light chains)

Product Code:
 
SwAR_IgG(H+L)
Product Group:
 
Secondary Antibody
Supplier:
 
Nordic MuBio
Host Type:
 
Porcine (Pig)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Storage:
 
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the product.
 

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CodeSizePrice
SwAR_IgG(H+L)1 ml£146.00
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This product comes from: Netherlands.
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Further Information

Applications Description:
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Background:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal human serum; as bridging reagent in a peroxidase antiperoxidase technique using R/PAP; to prepare an immunoadsorbent for the production of Ig-free rabbit serum, plasma or non-Ig preparations
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Product:
Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 ml sterile distilled water.
Source:
Purified normal IgG isolated from pooled rabbit serum. Freund?s complete adjuvant is used in the first step of the immunization procedure.
Specificity:
Precipitating polyclonal Swine antiSerum to Rabbit IgG, heavy and light chains.