NSJ Bioreagents

CHD4 Antibody / Chromodomain helicase DNA binding protein 4

Product Code:
 
NSJ-V7676
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3F2/4
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CHD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Immunofluorescent staining of permeabilized human MCF7 cells with CHD4 antibody (clone 3F2/4, green) and Phalloidin (red).
2 / 4
IHC staining of FFPE human breast carcinoma with CHD4 antibody (clone 3F2/4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 4
Western blot testing of human SK-BR-3 cells with CHD4 antibody. Predicted molecular weight ~260 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free CHD4 antibody (clone 3F2/4) as confirmation of integrity and purity.
❮❯

Immunofluorescent staining of permeabilized human MCF7 cells with CHD4 antibody (clone 3F2/4, green) and Phalloidin (red).
IHC staining of FFPE human breast carcinoma with CHD4 antibody (clone 3F2/4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human SK-BR-3 cells with CHD4 antibody. Predicted molecular weight ~260 kDa.
SDS-PAGE analysis of purified, BSA-free CHD4 antibody (clone 3F2/4) as confirmation of integrity and purity.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V7676-20UG20 ug£264.00
Quantity:
NSJ-V7676-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids ASGLGSPSPCSAGSEEEDM and CSRLANRAPEPPPQQVAQQQ were used as the immunogen for the CHD4 antibody.
Limitation:
This CHD4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
Q14839