Bromodeoxyuridine / BrdU Antibody

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Optimal dilution of the BrdU antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 min at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.

This BrdU antibody is available for research use only.

Nuclear

Protein G affinity chromatography

All species

Not Applicable