NSJ Bioreagents

Beta-Tubulin Antibody (TUBB)

Product Code:
 
NSJ-F40189
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, k
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
87CT59.3.7
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the beta-Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot analysis of lysate from 293, CEM, HeLa, Y79, rat PC-12 cell line, mouse brain tissue lysate using TUBB / beta-Tubulin antibody at 1:1000 for each lane.
2 / 4
Immunohistochemical analysis of paraffin-embedded human kidney using beta-Tubulin antibody at 1:25 dilution.
3 / 4
Immunofluorescent analysis of A549 cells using beta-Tubulin antibody at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight 554 (red) conjugated Phalloidin (red).
4 / 4
IHC analysis of FFPE human testis stained with beta-Tubulin antibody
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Western blot analysis of lysate from 293, CEM, HeLa, Y79, rat PC-12 cell line, mouse brain tissue lysate using TUBB / beta-Tubulin antibody at 1:1000 for each lane.
Immunohistochemical analysis of paraffin-embedded human kidney using beta-Tubulin antibody at 1:25 dilution.
Immunofluorescent analysis of A549 cells using beta-Tubulin antibody at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight 554 (red) conjugated Phalloidin (red).
IHC analysis of FFPE human testis stained with beta-Tubulin antibody

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CodeSizePrice
NSJ-F40189-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40189-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:25,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the beta-Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Tubulin beta chain recombinant protein was used to produce this beta-Tubulin antibody.
Limitation:
This beta-Tubulin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Rat, Bovine, Pig, Chicken, Hamster, Xenopus
Uniprot #:
P07437