NSJ Bioreagents

MGMT Antibody

Product Code:
 
NSJ-F52339
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
888CT22.2.4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Immunohistochemical analysis of paraffin-embedded human small intestine using MGMT antibody at 1:25 dilution.
2 / 3
Fluorescent image of HeLa cells stained with MGMT antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 3
MGMT antibody western blot analysis in MCF-7 lysate
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Immunohistochemical analysis of paraffin-embedded human small intestine using MGMT antibody at 1:25 dilution.
Fluorescent image of HeLa cells stained with MGMT antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
MGMT antibody western blot analysis in MCF-7 lysate

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CodeSizePrice
NSJ-F52339-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52339-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
IHC (Paraffin): 1:25,Immunofluorescence: 1:25,Western blot: 1:1000
Application Note:
Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified His-tagged protein was used to produced this monoclonal MGMT antibody.
Limitation:
This MGMT antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P16455