NSJ Bioreagents

ARF1 Antibody

Product Code:
 
NSJ-V7901
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ARF1/2117
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 6
IHC staining of FFPE human brain with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human liver with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human breast with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human kidney with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE rat brain with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
6 / 6
SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone ARF1/2117) as confirmation of integrity and purity.

IHC staining of FFPE human brain with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with ARF1 antibody (clone ARF1/2117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone ARF1/2117) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7901-20UG20 ug£264.00
Quantity:
NSJ-V7901-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant full-length human ARF1 protein was used as the immunogen for this ARF1 antibody.
Limitation:
This ARF1 antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P84077