NSJ Bioreagents

Aurora B Antibody / AURKB

Product Code:
 
NSJ-V7296
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
ARRK2-2R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant AURKB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human colon tissue with recombinant AURKB antibody (clone ARRK2-2R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human colon tissue with recombinant AURKB antibody (clone ARRK2-2R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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CodeSizePrice
NSJ-V7296-20UG20 ug£264.00
Quantity:
NSJ-V7296-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
IHC (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the recombinant AURKB antibody should be determined by the researcher.
Description:
Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 89-251 was used as the immunogen for this recombinant AURKB antibody.
Limitation:
This recombinant AURKB antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein A affinity
Species Reactivity :
Human
Uniprot #:
Q96GD4