NSJ Bioreagents

CD31 Antibody Cocktail / PECAM-1

Product Code:
 
NSJ-V2784
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C31.3 + C31.7 + C31.10
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD31 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC: Formalin-fixed, paraffin-embedded colon carcinoma stained with CD31 antibody cocktail (C31.3+C31.7+C31.10).~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with CD31 antibody cocktail (clones C31.3 + C31.7 + C31.1

IHC: Formalin-fixed, paraffin-embedded colon carcinoma stained with CD31 antibody cocktail (C31.3+C31.7+C31.10).~
IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with CD31 antibody cocktail (clones C31.3 + C31.7 + C31.1

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CodeSizePrice
NSJ-V2784-20UG20 ug£264.00
Quantity:
NSJ-V2784-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD31 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human recombinant protein was used as the immunogen for the CD31 antibody cocktail.
Limitation:
This CD31 antibody cocktail is available for research use only.
Localization:
Cell surface and cytoplasm of endothelial cells
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16284