NSJ Bioreagents

Calponin 1 Antibody Cocktail

Product Code:
 
NSJ-V2466
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CNN1/832 + CALP
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Calponin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded rat uterus stained with Calponin antibody (CNN1/832 + CALP).~
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IHC: Formalin-fixed, paraffin-embedded human uterus stained with Calponin antibody (CNN1/832 + CALP).

IHC: Formalin-fixed, paraffin-embedded rat uterus stained with Calponin antibody (CNN1/832 + CALP).~
IHC: Formalin-fixed, paraffin-embedded human uterus stained with Calponin antibody (CNN1/832 + CALP).

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CodeSizePrice
NSJ-V2466-20UG20 ug£264.00
Quantity:
NSJ-V2466-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.
Limitation:
This Calponin antibody cocktail is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P51911