NSJ Bioreagents

Nuclear Antigen Antibody

Product Code:
 
NSJ-V7192
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NUCM1-1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Nuclear Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE rat lung with Nuclear Antigen antibody (clone NUCM1-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.~
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IHC
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IHC testing of FFPE rat lung with Nuclear Antigen antibody (clone NUCM1-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.~
IHC

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CodeSizePrice
NSJ-V7192-20UG20 ug£264.00
Quantity:
NSJ-V7192-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the Nuclear Antigen antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
Limitation:
This Nuclear Antigen antibody is available for research use only.
Localization:
Nuclei
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
Not Known