NSJ Bioreagents

EIF2A Antibody

Product Code:
 
NSJ-F51764
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the EIF2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of EIF2A antibody and Ramos lysate. Predicted molecular weight: 62, 58, 41 kDa.
2 / 3
IHC analysis of FFPE human brain tissue stained with EIF2A antibody
3 / 3
EIF2A antibody flow cytometry analysis of Ramos cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of EIF2A antibody and Ramos lysate. Predicted molecular weight: 62, 58, 41 kDa.
IHC analysis of FFPE human brain tissue stained with EIF2A antibody
EIF2A antibody flow cytometry analysis of Ramos cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F51764-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51764-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the EIF2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EIF2A functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 437-465 from the human protein was used as the immunogen for this EIF2A antibody.
Limitation:
This EIF2A antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9BY44