NSJ Bioreagents

ISG15 Antibody

Product Code:
 
NSJ-F41498
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the ISG15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC analysis of FFPE human breast carcinoma tissue stained with the ISG15 antibody
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Western blot analysis of ISG15 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (2).
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ISG15 antibody western blot analysis in 293 lysate
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ISG15 antibody western blot analysis in Jurkat, K562 and AML-2 lysate treated with CQ (0/10/20/30uM) for 24 hours. (Courtesy of Biyin Cao, Soochow University, Center for Blood Research)
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IHC analysis of FFPE human breast carcinoma tissue stained with the ISG15 antibody
Western blot analysis of ISG15 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (2).
ISG15 antibody western blot analysis in 293 lysate
ISG15 antibody western blot analysis in Jurkat, K562 and AML-2 lysate treated with CQ (0/10/20/30uM) for 24 hours. (Courtesy of Biyin Cao, Soochow University, Center for Blood Research)

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CodeSizePrice
NSJ-F41498-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41498-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
IHC (Paraffin): 1:50-1:100,Western blot: 1:1000
Application Note:
Titration of the ISG15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 31-61 from the human protein was used as the immunogen for this ISG15 antibody.
Limitation:
This ISG15 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P05161