NSJ Bioreagents

Histone H4 Antibody

Product Code:
 
NSJ-F52511
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the Histone H4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Immunohistochemical analysis of paraffin-embedded human skin section using Histone H4 antibody diluted at 1:25 dilution.
2 / 7
Immunohistochemical analysis of paraffin-embedded human esophagus section using Histone H4 antibody diluted at 1:25 dilution.
3 / 7
Immunohistochemical analysis of paraffin-embedded mouse skin section using Histone H4 antibody diluted at 1:25 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse esophagus section using Histone H4 antibody diluted at 1:25 dilution.
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Immunohistochemical analysis of paraffin-embedded rat skin section using Histone H4 antibody diluted at 1:25 dilution.
6 / 7
Western blot analysis of lysate from (1) A431, (2) HeLa, (3) H-4-II-E and (4) L929 cell lines using Histone H4 antibody at 1:1000.
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Flow cytometric analysis of MCF-7 cells using Histone H4 antibody (green) compared to an <a href=../search_result.php?search_txt=n1001>isotype control of rabbit IgG</a> (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
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Immunohistochemical analysis of paraffin-embedded human skin section using Histone H4 antibody diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded human esophagus section using Histone H4 antibody diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded mouse skin section using Histone H4 antibody diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded mouse esophagus section using Histone H4 antibody diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded rat skin section using Histone H4 antibody diluted at 1:25 dilution.
Western blot analysis of lysate from (1) A431, (2) HeLa, (3) H-4-II-E and (4) L929 cell lines using Histone H4 antibody at 1:1000.
Flow cytometric analysis of MCF-7 cells using Histone H4 antibody (green) compared to an <a href=../search_result.php?search_txt=n1001>isotype control of rabbit IgG</a> (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.

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CodeSizePrice
NSJ-F52511-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52511-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow Cytometry: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
Application Note:
Titration of the Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This Histone H4 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 71-103 amino acids from the C-terminal region of human HIST1H4A.
Limitation:
This Histone H4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Bovine, C. elegans, Chicken, Drosophila, Pig, Primate, Rat, Xenopus, Yeast
Uniprot #:
P62805