NSJ Bioreagents

DIEXF Antibody / DEF

Product Code:
 
NSJ-F54357
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the DIEXF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HT-1080, 2) K562 and 3) RPMI 8226 cell lysate with DIEXF antibody. Predicted molecular weight ~87 kDa.
2 / 4
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with DIEXF antibody; Blue=isotype control, Green= DIEXF antibody.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with DIEXF antibody (green) and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human hepatocarcinoma with DIEXF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HT-1080, 2) K562 and 3) RPMI 8226 cell lysate with DIEXF antibody. Predicted molecular weight ~87 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with DIEXF antibody; Blue=isotype control, Green= DIEXF antibody.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with DIEXF antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human hepatocarcinoma with DIEXF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54357-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54357-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the DIEXF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Regulates the p53 pathway to control the expansion growth of digestive organs (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 2-30 from the human protein was used as the immunogen for the DIEXF antibody .
Limitation:
This DIEXF antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q68CQ4