NSJ Bioreagents

HLA-DRB1 Antibody

Product Code:
 
NSJ-F54895
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the HLA-DRB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human 1) NCI-H1299 and 2) Raji cell lysate with HLA-DRB1 antibody. Predicted molecular weight ~30 kDa.
2 / 3
IHC testing of FFPE human tonsil tissue with HLA-DRB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Flow cytometry testing of human WiDr cells with HLA-DRB1 antibody; Blue=isotype control, Green= HLA-DRB1 antibody.
❮❯

Western blot testing of human 1) NCI-H1299 and 2) Raji cell lysate with HLA-DRB1 antibody. Predicted molecular weight ~30 kDa.
IHC testing of FFPE human tonsil tissue with HLA-DRB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with HLA-DRB1 antibody; Blue=isotype control, Green= HLA-DRB1 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54895-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54895-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the HLA-DRB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 30-58 from the human protein was used as the immunogen for the HLA-DRB1 antibody.
Limitation:
This HLA-DRB1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04229