NSJ Bioreagents

IDOL Antibody / MYLIP

Product Code:
 
NSJ-F54294
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the IDOL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human 1) 293 and 2) MCF7 cell lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
2 / 5
Western blot testing of mouse kidney lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
3 / 5
Western blot testing of rat brain lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
4 / 5
IHC testing of FFPE human brain tissue with IDOL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human MCF7 cells with IDOL antibody; Blue=isotype control, Green= IDOL antibody.
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Western blot testing of human 1) 293 and 2) MCF7 cell lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
Western blot testing of mouse kidney lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
Western blot testing of rat brain lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human brain tissue with IDOL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with IDOL antibody; Blue=isotype control, Green= IDOL antibody.

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CodeSizePrice
NSJ-F54294-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54294-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the IDOL antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP), also called Inducible degrader of the LDL-receptor (IDOL), is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 111-139 from the human protein were used as the immunogen for the IDOL antibody.
Limitation:
This IDOL antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8WY64