NSJ Bioreagents

RAB1B Antibody

Product Code:
 
NSJ-F54676
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the RAB1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HEK293, 2) A549, 3) Hela, 4) T-47D, 5) U-87 MG, 6) mouse liver and 7) rat liver lysate with RAB1B antibody. Predicted molecular weight ~22 kDa.
2 / 4
Western blot testing of human 1) HEK293, 2) A549, 3) Hela, 4) T-47D, 5) U-87 MG, 6) mouse NIH 3T3 and 7) rat liver lysate with RAB1B antibody. Predicted molecular weight ~22 kDa.
3 / 4
IHC testing of FFPE human kidney tissue with RAB1B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
4 / 4
IHC testing of FFPE human liver tissue with RAB1B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HEK293, 2) A549, 3) Hela, 4) T-47D, 5) U-87 MG, 6) mouse liver and 7) rat liver lysate with RAB1B antibody. Predicted molecular weight ~22 kDa.
Western blot testing of human 1) HEK293, 2) A549, 3) Hela, 4) T-47D, 5) U-87 MG, 6) mouse NIH 3T3 and 7) rat liver lysate with RAB1B antibody. Predicted molecular weight ~22 kDa.
IHC testing of FFPE human kidney tissue with RAB1B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with RAB1B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54676-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54676-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the RAB1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi.[OMIM].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 166-194 from the human protein was used as the immunogen for the RAB1B antibody.
Limitation:
This RAB1B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H0U4