NSJ Bioreagents

MEK4 Antibody / MKK4 / MAP2K4

Product Code:
 
NSJ-F54701
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Zebrafish
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the MEK4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human Y79 cell lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
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Western blot testing of zebrafish brain lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
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Western blot testing of mouse NIH 3T3 cell lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
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IHC testing of FFPE mouse cerebellum tissue with MEK4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human Y79 cell lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
Western blot testing of zebrafish brain lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with MEK4 antibody. Predicted molecular weight ~44 kDa.
IHC testing of FFPE mouse cerebellum tissue with MEK4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54701-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54701-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the MEK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 259-287 from the mouse protein was used as the immunogen for the MEK4 antibody.
Limitation:
This MEK4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Zebrafish
Uniprot #:
P47809