NSJ Bioreagents

SDHA Antibody

Product Code:
 
NSJ-RQ5669
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SDHA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of human 1) HeLa, 2) HepG2, 3) HEK293, 4) Caco-2 and rat 5) brain, 6) kidney, 7) spleen, 8) liver and mouse 9) brain, 10) kidney, 11) liver lysate with SDHA antibody. Predicted molecular weight ~72 kDa.
2 / 5
Immunofluorescent staining of FFPE human HeLa cells with SDHA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
Flow cytometry testing of human HeLa cells with SDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDHA antibody.
4 / 5
Flow cytometry testing of mouse RAW264.7 cells with SDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDHA antibody.
5 / 5
Flow cytometry testing of rat C6 cells with SDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDHA antibody.
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Western blot testing of human 1) HeLa, 2) HepG2, 3) HEK293, 4) Caco-2 and rat 5) brain, 6) kidney, 7) spleen, 8) liver and mouse 9) brain, 10) kidney, 11) liver lysate with SDHA antibody. Predicted molecular weight ~72 kDa.
Immunofluorescent staining of FFPE human HeLa cells with SDHA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HeLa cells with SDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDHA antibody.
Flow cytometry testing of mouse RAW264.7 cells with SDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDHA antibody.
Flow cytometry testing of rat C6 cells with SDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDHA antibody.

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NSJ-RQ5669-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the SDHA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Succinate dehydrogenase complex, subunit A, flavoprotein variant is a protein that in humans is encoded by the SDHA gene. It is mapped to 5p15.33. This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids YRPVIDKTLNEADCAT from the human protein were used as the immunogen for the SDHA antibody.
Limitation:
This SDHA antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P31040