NSJ Bioreagents

SOX6 Antibody

Product Code:
 
NSJ-RQ5840
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SOX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse testis with SOX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat testis with SOX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) placenta, 2) Caco-2 and 3) U-2 OS lysate with SOX6 antibody. Predicted molecular weight ~92 kDa.
4 / 4
Flow cytometry testing of human A549 cells with SOX6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SOX6 antibody.
❮❯

IHC staining of FFPE mouse testis with SOX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with SOX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) Caco-2 and 3) U-2 OS lysate with SOX6 antibody. Predicted molecular weight ~92 kDa.
Flow cytometry testing of human A549 cells with SOX6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SOX6 antibody.

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CodeSizePrice
NSJ-RQ5840-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SOX6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Transcription factor SOX-6 is a protein that in humans is encoded by the SOX6 gene. This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DQDTVMKAIQEARKMREQIQREQQQQ from the human protein were used as the immunogen for the SOX6 antibody.
Limitation:
This SOX6 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P35712