NSJ Bioreagents

MDM2 Antibody

Product Code:
 
NSJ-RQ5828
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the MDM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Western blot testing of 1) human U-87 MG, 2) rat PC-12, 3) rat RH35, 4) mouse HEPA1-6, 5) mouse NIH 3T3 and 6) mouse ANA-1 lysate with MDM2 antibody. Predicted molecular weight: ~55 kDa but can be observed at up to ~90 kDa.
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Western blot testing of 1) human U-87 MG, 2) rat PC-12, 3) rat RH35, 4) mouse HEPA1-6, 5) mouse NIH 3T3 and 6) mouse ANA-1 lysate with MDM2 antibody. Predicted molecular weight: ~55 kDa but can be observed at up to ~90 kDa.

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CodeSizePrice
NSJ-RQ5828-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the MDM2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QYIMTKRLYDEKQQHIVY from the human protein were used as the immunogen for the MDM2 antibody.
Limitation:
This MDM2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q00987