NSJ Bioreagents

SMARCAD1 Antibody

Product Code:
 
NSJ-RQ5189
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the SMARCAD1 antibody at -20oC.
1 / 8
Western blot testing of human 1) K562, 2) HL-60, 3) U-2 OS, 4) U-87 MG, 5) HepG2, 6) ThP-1 and 7) A549 lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.
2 / 8
Western blot testing of 1) rat testis and mouse 2) thymus, 3) lung, 4) testis and 5) kidney tissue lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.
3 / 8
Immunofluorescent staining of FFPE human A431 cells with SMARCAD1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 8
IHC staining of FFPE human breast cancer with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human colon cancer with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE mouse brain with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 8
IHC staining of FFPE rat brain with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 8
Flow cytometry testing of human SiHa cells with SMARCAD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMARCAD1 antibody.
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Western blot testing of human 1) K562, 2) HL-60, 3) U-2 OS, 4) U-87 MG, 5) HepG2, 6) ThP-1 and 7) A549 lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.
Western blot testing of 1) rat testis and mouse 2) thymus, 3) lung, 4) testis and 5) kidney tissue lysate with SMARCAD1 antibody. Predicted molecular weight ~117 kDa but can be observed at up to ~150 kDa.
Immunofluorescent staining of FFPE human A431 cells with SMARCAD1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with SMARCAD1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human SiHa cells with SMARCAD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMARCAD1 antibody.

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CodeSizePrice
NSJ-RQ5189-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml, Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SMARCAD1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 is a protein that in humans is encoded by the SMARCAD1 gene. It is mapped to 4q22.3. This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids D827-T1002 from the human protein were used as the immunogen for the SMARCAD1 antibody.
Limitation:
This SMARCAD1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H4L7