ELK Biotechnology

Human NES1(Nesfatin 1) ELISA Kit

Product Code:
 
ELK2828
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK2828-48T48T£298.00
Quantity:
ELK2828-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
Nesfatin-1
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
781.25-50000 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
234.2 pg/mL
Standard:
50000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NES1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.