ELK Biotechnology

Rat SOX2(Sex Determining Region Y Box Protein 2) ELISA Kit

Product Code:
 
ELK2978
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK2978-48T48T£298.00
Quantity:
ELK2978-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
ANOP3; MCOPS3; SRY-Box 2; Transcription factor SOX-2; SRY related HMG box gene 2; SRY related HMG box 2
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
0.064 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SOX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SOX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SOX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat SOX2 in the samples is then determined by comparing the OD of the samples to the standard curve.