ELK Biotechnology

Rat EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit

Product Code:
 
ELK5785
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK5785-48T48T£298.00
Quantity:
ELK5785-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
CD340; ErbB2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.63-40 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
0.221 ng/mL
Standard:
40 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.