ELK Biotechnology

Sheep LEP(Leptin Receptor) ELISA Kit

Product Code:
 
ELK8296
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Sheep
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8296-48T48T£359.00
Quantity:
ELK8296-96T96T£477.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.054 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sheep LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sheep LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sheep LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of sheep LEP in the samples is then determined by comparing the OD of the samples to the standard curve.