ELK Biotechnology

General CNCbl(Cyanocobalamin) ELISA Kit

Product Code:
 
ELK7871
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK7871-48T48T£261.00
Quantity:
ELK7871-96T96T£335.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
156.25-10000 pg/mL
Sample Type:
serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
49.2 pg/mL
Standard:
10000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNCbl protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.