ELK Biotechnology

Guinea pig VF(Visfatin) ELISA Kit

Product Code:
 
ELK7097
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Cavia (Guinea pig)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK7097-48T48T£359.00
Quantity:
ELK7097-96T96T£477.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
1.57-100 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
0.52 ng/mL
Standard:
100 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Guinea pig VF in the samples is then determined by comparing the OD of the samples to the standard curve.