Fc-KIH (human) IgG1 Control (rec.)
| Code | Size | Price |
|---|
| AG-35B-0015-C010 | 10 ug | £290.00 |
Quantity:
| AG-35B-0015-3010 | 3 x 10 ug | £570.00 |
Quantity:
| AG-35B-0015-C100 | 100 ug | £1,130.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Shipping:
BLUE ICE
Storage:
Short Term Storage: +4?C. Long Term Storage: -20?C
Documents
Further Information
Alternate Names/Synonyms:
Fc Knobs-into-Holes (human) IgG1; Immunoglobulin 1 Fragment Constant
Concentration:
After reconstitution: for 10?g size: 0.1mg/ml, for 100?g size: 1mg/ml
EClass:
32160000
Endotoxin:
<0.01EU/?g purified protein (LAL test).
Form (Short):
solid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20?C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Recombinant Protein. Fc (human) IgG1 (knobs-into-holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996). Source: HEK 293 cells. Lyophilized. Contains PBS. Negative control in experiments with recombinant proteins fused to human IgG1 Fc-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0221). This protein is not an appropriate negative control for other standard Fc protein constructs. The protein Fc-KIH (human) IgG1 Control (rec.) is produced by using two different vectors, one encoding for the Fc Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc-KIH (human) IgG1 Control (rec.).
Molecular Weight:
~28kDa (SDS-PAGE)
Package Type:
Plastic Vial
Product Description:
Negative control in experiments with recombinant proteins fused to human IgG1 Fc-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0221). This protein is not an appropriate negative control for other standard Fc protein constructs. The protein Fc-KIH (human) IgG1 Control (rec.) is produced by using two different vectors, one encoding for the Fc Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc-KIH (human) IgG1 Control (rec.).
Purity:
>95% (SDS-PAGE)
Sequence:
Fc (human) IgG1 (knobs-into-holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996).
Source / Host:
HEK 293 cells
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20?C. Working aliquots are stable for up to 3 months when stored at -20?C.