NSJ Bioreagents

NME7 Antibody / Nucleoside diphosphate kinase 7

Product Code:
 
NSJ-F54961
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the NME7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human T-47D cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
2 / 4
Western blot testing of human A549 cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NME7 antibody.
4 / 4
IHC testing of FFPE human cancer tissue with NME7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Western blot testing of human T-47D cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human A549 cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NME7 antibody.
IHC testing of FFPE human cancer tissue with NME7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54961-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54961-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 25-55 from the human protein was used as the immunogen for the NME7 antibody.
Limitation:
This NME7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9Y5B8