NSJ Bioreagents

VPS53 Antibody

Product Code:
 
NSJ-F54974
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the VPS53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of mouse brain tissue lysate with VPS53 antibody. Predicted molecular weight ~94 kDa.
2 / 4
Western blot testing of human MCF7 cell lysate with VPS53 antibody. Predicted molecular weight ~94 kDa.
3 / 4
IHC testing of FFPE human brain tissue with VPS53 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MDA-MB-468 cells with VPS53 antibody; Blue=isotype control, Green= VPS53 antibody.
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Western blot testing of mouse brain tissue lysate with VPS53 antibody. Predicted molecular weight ~94 kDa.
Western blot testing of human MCF7 cell lysate with VPS53 antibody. Predicted molecular weight ~94 kDa.
IHC testing of FFPE human brain tissue with VPS53 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-468 cells with VPS53 antibody; Blue=isotype control, Green= VPS53 antibody.

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CodeSizePrice
NSJ-F54974-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54974-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
VPS53 is a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 111-140 from the human protein was used as the immunogen for the VPS53 antibody.
Limitation:
This VPS53 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q5VIR6