NSJ Bioreagents

Bim Antibody / BCL2L11

Product Code:
 
NSJ-F54977
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Bcl-2-like protein 11 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human colon carcinoma tissue with Bcl-2-like protein 11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with Bcl-2-like protein 11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) K562 and 2) HL60 cell lysate with Bcl-2-like protein 11 antibody. Predicted molecular weight ~22 kDa.
4 / 4
Flow cytometry testing of human WiDr cells with Bcl-2-like protein 11 antibody; Blue=isotype control, Green= Bcl-2-like protein 11 antibody.
❮❯

IHC testing of FFPE human colon carcinoma tissue with Bcl-2-like protein 11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Bcl-2-like protein 11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) K562 and 2) HL60 cell lysate with Bcl-2-like protein 11 antibody. Predicted molecular weight ~22 kDa.
Flow cytometry testing of human WiDr cells with Bcl-2-like protein 11 antibody; Blue=isotype control, Green= Bcl-2-like protein 11 antibody.

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CodeSizePrice
NSJ-F54977-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54977-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:50-1:100 (1x10e6 cells), Western blot: 1:500-1:1000
Description:
Bcl-2-like protein 11, also called BCL2L11 and Bim, belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 134-160 from the human protein was used as the immunogen for the Bcl-2-like protein 11 antibody.
Limitation:
This Bcl-2-like protein 11 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O43521